The Marine Biological Laboratory The Josephine Bay Paul Center
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VAMPS
Amplification Primers


Primers
We use primers that are a combination of the Roche A- and B-adaptors for promoting sequencing, a 5 nucleotide key for identifying samples within a mixture of samples and the amplification primers.

Complete Description of Our Primers


5-Nucleotide Keys
We use nucleotide "keys" embedded within the amplification primers to differentiate multiple samples on a GS-FLX run without physically partitioning the picotiter plate. We currently use 5-nucleotide keys, although longer or shorter keys can also work.

Complete Listing of Our Keys


 
 
     
Supported by Alfred P. Sloan Foundation and the Josephine Bay Paul and C. Michael Paul Foundation.
Unless otherwise stated, all material © 2007 Bay Paul Center, MBL.