Primers
We use primers that are a combination of the Roche A- and B-adaptors for promoting sequencing, a 5 nucleotide key for identifying samples within a mixture of samples and the amplification primers.

Complete Description of Our Primers

5-Nucleotide Keys
We use nucleotide "keys" embedded within the amplification primers to differentiate multiple samples on a GS-FLX run without physically partitioning the picotiter plate. We currently use 5-nucleotide keys, although longer or shorter keys can also work.

Complete Listing of Our Keys