sequenceserver_logo 2.0.0
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Advanced Options

General Search Options
  • -task <String>
      Task to execute for the selected BLAST algorithm
      Options for BLASTN:
        'blastn'       Traditional BLASTN requiring an exact match of 11 (default)
        'blastn-short' Optimized BLASTN for query sequences shorter than 50 nucleotides.
        'megablast'    Traditional megablast used to find very similar sequences (e.g., intraspecies or closely related species).
        'dc-megablast' Discontiguous megablast used to find somewhat distant sequences (e.g., interspecies).
        'rmblastn'
      Options for BLASTP:
        'blastp'       Standard protein-protein comparisons (default)
        'blastp-fast'  Faster BLASTP searches using longer words for protein seeding
        'blastp-short' Optimized BLASTP comparisons for query sequences shorter than 30 residues
      Options for BLASTX:
        'blastx'       Standard nucleotide-protein comparisons (default)
        'blastx-fast'  Optimized nucleotide-protein comparisons
      Options for TBLASTN:
        'tblastn'      Standard protein-nucleotide comparisons (default)
        'tblastn-fast' Optimized protein-nucleotide comparisons
      Not applicable for TBLASTX
  • -evalue <Real>
      Expectation value (E) threshold for saving hits
      Default = '10'
  • -word_size <Integer, >=2>
      Word size for wordfinder algorithm
  • -gapopen <Integer>
      Cost to open a gap
  • -gapextend <Integer>
      Cost to extend a gap
  • -matrix <String>
      Scoring matrix name (normally BLOSUM62)
  • -threshold <Real, >=0>
      Minimum word score such that the word is added to the BLAST lookup
      table
  • -comp_based_stats <String>
      Use composition-based statistics for blastp / tblastn:
          D or d: default (equivalent to 2)
          0 or F or f: no composition-based statistics
          1: Composition-based statistics as in NAR 29:2994-3005, 2001
          2 or T or t : Composition-based score adjustment as in
          Bioinformatics 21:902-911, 2005, conditioned on sequence
          properties
          3: Composition-based score adjustment as in Bioinformatics
          21:902-911, 2005, unconditionally
      For programs other than tblastn, must either be absent or be D, F
      or 0
      Default = `2'
  • -num_descriptions <Integer, >=0>
      Number of database sequences to show one-line descriptions for
      Default = `500'
  •  -num_alignments <Integer, >=0>
      Number of database sequences to show alignments for
      Default = `250'

Query filtering options
  • -seg <String>
      Filter query sequence with SEG (Format: 'yes', 'window locut hicut',
      or 'no' to disable)
      Default = `no'
  • -soft_masking <Boolean>
      Apply filtering locations as soft masks
      Default = `false'
  • -lcase_masking
      Use lower case filtering in query and subject sequence(s)?

Restrict search or results
  • -gilist <String>
      Restrict search of database to list of GI's
        * Incompatible with:  negative_gilist, seqidlist, remote, subject,
      subject_loc
  • -seqidlist <String>
      Restrict search of database to list of SeqId's
        * Incompatible with:  gilist, negative_gilist, remote, subject,
      subject_loc
  • -negative_gilist <String>
      Restrict search of database to everything except the listed GIs
        * Incompatible with:  gilist, seqidlist, remote, subject,
          subject_loc
  • -entrez_query <String>
      Restrict search with the given Entrez query
        * Requires:  remote
  • -db_soft_mask <String>
      Filtering algorithm ID to apply to the BLAST database as soft masking
        * Incompatible with:  subject, subject_loc
  • -culling_limit <Integer, >=0>
      If the query range of a hit is enveloped by that of at least this
      many higher-scoring hits, delete the hit
        * Incompatible with:  best_hit_overhang, best_hit_score_edge
  • -best_hit_overhang <Real, (>0 and <0.5)>
      Best Hit algorithm overhang value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -best_hit_score_edge <Real, (>0 and <0.5)>
      Best Hit algorithm score edge value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -max_target_seqs <Integer, >=1>
      Maximum number of aligned sequences to keep

Please cite data sources and the paper describing our SequenceServer BLAST interface. Perform BLAST analyses.

Advanced Options

General Search Options
  • -task <String>
      Task to execute for the selected BLAST algorithm
      Options for BLASTN:
        'blastn'       Traditional BLASTN requiring an exact match of 11 (default)
        'blastn-short' Optimized BLASTN for query sequences shorter than 50 nucleotides.
        'megablast'    Traditional megablast used to find very similar sequences (e.g., intraspecies or closely related species).
        'dc-megablast' Discontiguous megablast used to find somewhat distant sequences (e.g., interspecies).
        'rmblastn'
      Options for BLASTP:
        'blastp'       Standard protein-protein comparisons (default)
        'blastp-fast'  Faster BLASTP searches using longer words for protein seeding
        'blastp-short' Optimized BLASTP comparisons for query sequences shorter than 30 residues
      Options for BLASTX:
        'blastx'       Standard nucleotide-protein comparisons (default)
        'blastx-fast'  Optimized nucleotide-protein comparisons
      Options for TBLASTN:
        'tblastn'      Standard protein-nucleotide comparisons (default)
        'tblastn-fast' Optimized protein-nucleotide comparisons
      Not applicable for TBLASTX
  • -evalue <Real>
      Expectation value (E) threshold for saving hits
      Default = '10'
  • -word_size <Integer, >=2>
      Word size for wordfinder algorithm
  • -gapopen <Integer>
      Cost to open a gap
  • -gapextend <Integer>
      Cost to extend a gap
  • -matrix <String>
      Scoring matrix name (normally BLOSUM62)
  • -threshold <Real, >=0>
      Minimum word score such that the word is added to the BLAST lookup
      table
  • -comp_based_stats <String>
      Use composition-based statistics for blastp / tblastn:
          D or d: default (equivalent to 2)
          0 or F or f: no composition-based statistics
          1: Composition-based statistics as in NAR 29:2994-3005, 2001
          2 or T or t : Composition-based score adjustment as in
          Bioinformatics 21:902-911, 2005, conditioned on sequence
          properties
          3: Composition-based score adjustment as in Bioinformatics
          21:902-911, 2005, unconditionally
      For programs other than tblastn, must either be absent or be D, F
      or 0
      Default = `2'
  • -num_descriptions <Integer, >=0>
      Number of database sequences to show one-line descriptions for
      Default = `500'
  •  -num_alignments <Integer, >=0>
      Number of database sequences to show alignments for
      Default = `250'

Query filtering options
  • -seg <String>
      Filter query sequence with SEG (Format: 'yes', 'window locut hicut',
      or 'no' to disable)
      Default = `no'
  • -soft_masking <Boolean>
      Apply filtering locations as soft masks
      Default = `false'
  • -lcase_masking
      Use lower case filtering in query and subject sequence(s)?

Restrict search or results
  • -gilist <String>
      Restrict search of database to list of GI's
        * Incompatible with:  negative_gilist, seqidlist, remote, subject,
      subject_loc
  • -seqidlist <String>
      Restrict search of database to list of SeqId's
        * Incompatible with:  gilist, negative_gilist, remote, subject,
      subject_loc
  • -negative_gilist <String>
      Restrict search of database to everything except the listed GIs
        * Incompatible with:  gilist, seqidlist, remote, subject,
          subject_loc
  • -entrez_query <String>
      Restrict search with the given Entrez query
        * Requires:  remote
  • -db_soft_mask <String>
      Filtering algorithm ID to apply to the BLAST database as soft masking
        * Incompatible with:  subject, subject_loc
  • -culling_limit <Integer, >=0>
      If the query range of a hit is enveloped by that of at least this
      many higher-scoring hits, delete the hit
        * Incompatible with:  best_hit_overhang, best_hit_score_edge
  • -best_hit_overhang <Real, (>0 and <0.5)>
      Best Hit algorithm overhang value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -best_hit_score_edge <Real, (>0 and <0.5)>
      Best Hit algorithm score edge value (recommended value: 0.1)
        * Incompatible with:  culling_limit
  • -max_target_seqs <Integer, >=1>
      Maximum number of aligned sequences to keep